ABSTRACT
Plants are invaluable sources of pharmaceutical products and Brazil, in particular, has yielded an incredible array of plant and animal products that have drawn the attention of ethnopharmacologists from around the world. Ouratea is a genus of tropical plants within the Ochnaceae. Ouratea is widespread throughtout the tropics, with species in Costa Rica, Brazil, Jamaica, and Kenya. The search for compounds with antimicrobial activity is urgent and indispensable for the treatment of infectious diseases caused by microorganisms resistant to traditional antimicrobial drugs. A screening was conducted with organic extracts from two species, from roots and leaves of Ouratea hexasperma var. planchonii Engl. and leaves and branches of Ouratea parviflora Engl., to identify plant extracts with antifungal properties against Candida albicans. Initially, minimal inhibitory concentrations (MICs) were determined in C. albicans cellular suspension in Sabouraud medium, in a range of 0.060-3.0 mg/ml. Fractions OHRMWE (Ethyl acetate fraction) and OHLMWB (Buthanol fraction) obtained by solvents partition of methanol:water extract from Ouratea hexasperma with ethyl acetate from the roots and n-butanol from the leaves, respectively, showed a higher antifungal activity. The antifungal activity of these extracts was also compared in growth delay of C. albicans cultures.
ABSTRACT
From the hexanic extract of the stem from Swartzia apetala Raddi var. glabra were isolated one stilbene (1), one flavanone (2), one pterocarpan (3), one triterpene (4) and a mixture of three steroids (5 to 7). The crude extract and the compounds isolated were submitted to evaluation of the antifungal activity against nine yeast standard ATCC of the Candida genus. Among the compounds only the triterpene (4) and the mixture of steroids (5 to 7) showed no activity. The structures of the compounds were determined by spectral data analysis of GC/MS and ¹H and 13C NMR (1D and 2D experiments), as well as comparison with literature values.
Do extrato hexânico da madeira de Swartzia apetala Raddi var. glabra foram isolados um estilbeno (1), uma flavanona (2), um pterocarpano (3), um triterpeno (4) e uma mistura de esteróides (5 a 7). O extrato bruto e as substâncias isoladas foram submetidas à avaliação do potencial antifúngico usando nove cepas padrão ATCC do gênero Candida. Entre as substâncias testadas apenas o triterpeno (4) e a mistura de esteróides (5 a 7) não apresentaram atividade. As estruturas das substâncias foram determinadas através da análise dos espectros de CG/EM, e RMN (1D e 2D) e comparação com dados da literatura.